Integrating Multiscale Modeling and in vivo Experiments for Studying Blood Clot Development

The overall goal of this project is to develop three-dimensional multi-scale mathematical models and a computational toolkit for simulating thrombus formation. These models will be validated with specifically designed experiments to test predictions of thrombus development, structure, and stability. Moreover, the development of reasonable models will serve as a generator of new hypotheses that can be tested in experiments in vivo.

Collaborators

  • Drs. Mark Alber, Zhiliang Xu, and Danny Chen (ACMS and Department of Computer Science and Engineering, Notre Dame)
  • Dr. Elliot Rosen (Medical and Molecular Genetics, Indiana University School of Medicine)
  • Dr. Alisa Wolberg (Pathology and Laboratory Medicine, UNC School of Medicine)
  • Joshua Lioi and Dr. Santanu Chatterjee (ACMS graduate student and Department of Computer Science and Engineering, Notre Dame)
  • Eunjung Kim (ACMS postdoc associate, Notre Dame)

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Click on the slide below to begin the simulation:

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Click on the slide below to begin the simulation:

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Three-dimensional images computationall reconstructed from two-dimensional
confocal microscopy scans of blood clots formed in a healthy mouse and in a
mouse with low levels of FVII (hemophiliac) (after 13 min).


Click on the below image to begin the rolling platelet simulation:

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Reconstructed three-dimensional structure of a fiber gel grown in a micro fluidics device.

 

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Analysis of the blood flow near the two clots using Lagrangian Coherent Structures (LCS)


 

Latest publications:

Xu, Z., M.M. Kamocka, M.S. Alber, and E.D. Rosen [2011], Computational Approaches to Studying Thrombus Development, Arterioscler Thromb Vasc Biol 31, 500-505.

Eungjun Kim, Oleg V. Kim, Kellie R. Machlus, Xiaomin Liu,Timur Kupaev, Joshua Lioi, Alisa S.Wolberg, Danny Z. Chen, Elliot D. Rosen, Zhiliang Xu, and Mark Alber [2011], Correlation between fibrin network structure and mechanical properties: an experimental and computational analysis, Soft Matter (Royal Chemical Society) 7, 4983.

Christopher R. Sweet, Santanu Chatterjee, Zhiliang Xu,Katharine Bisordi, Elliot D. Rosen and Mark Alber, Modeling Platelet-Blood Flow Interaction Using Subcellular Element Langevin Method,Modeling Platelet-Blood Flow Interaction Using Subcellular Element Langevin Method, Journal of the Royal Society Interface,  (in press).

Xu, Z., J. Lioi, J. Mu, X. Liu, M.M. Kamocka, E.D. Rosen, D.Z. Chen and M.S. Alber [2010], A Multiscale Model of Venous Thrombus Formation with Surface-Mediated Control of Blood Coagulation Cascade, Biophysical Journal 98, 9, 1723–1732.